A new H-2.1-PositiveD region allele,D dx,controlling two molecules,H-2Ddx and H-2Ldx |
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Authors: | Margriet Snoek Dagmar Iványi Roeland Nusse Peter Démant |
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Affiliation: | (1) Division of Genetics, Antoni van Leeuwenhoekhuis, The Netherlands Cancer Institute, Amsterdam, The Netherlands |
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Abstract: | The inbred strains GRS/A and LIS/A carry the haplotypeH-2dx, which had earlier been shown to have theKd,If,Sf, andGf alleles and a previously unknownD region allele,Ddx. We show here that theDdx allele determines a new private specificity, H-2.63, is H-2.28 negative, and determines at least one public specificity of the H-2.1 family. It is thus a second example (afterDk) of a H-2.1-positive H-2.28-negativeD region allele. Capping experiments show that the Ddx product comprises two molecules: H-2Ddx bearing the private specificity H-2.63, and H-2Ldx, which is H-2.63-negative but reacts with sera against the H-2.1 family of specificities. SDS gel electrophoresis of detergent-solubilized immunoprecipitated Ddx products shows that the H-2Ldx antigen has a molecular weight of approximately 45,000 daltons and is associated with a smaller polypeptide (mol. wt. 12,000). |
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