Separation By Velocity Sedimentation of Human Haemopoietic Precursors Forming Colonies in vivo and in vitro Cultures |
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| Authors: | E Niskanen J R Wells D W Golde M J Cline |
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| Institution: | Department of Internal Medicine, University of Virginia School of Medicine, Charlottesville, Virginia 22908, and Department of Medicine, UCLA School of Medicine, Los Angeles, California 90024, U. S. A. |
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| Abstract: | Cells which give rise to granulocyte-macrophage colonies under the influence of peripheral blood white cells (CFU-c (WBC)) and Mo T cell conditioned medium (CFU-c (Mo)) sedimented at a faster rate than the cells which form mixed erythroid-granulocytic colonies in methylcellulose in vitro (CFU-mix) and granulocytic (CFU-dg) and megakaryocytic (CFU-dm) colonies in diffusion chambers in mice. Despite identical peak sedimentation rate for the two CFU-c populations, sedimentation profiles suggest that they are heterogeneous with respect to size. A proportion of CFU-c (Mo) may be identical with CFU-dg and CFU-mix. Sedimentation profiles for cells which give rise to mixed colonies in vitro (CFU-mix) and to granulocytic colonies in diffusion chambers in cyclophosphamide pretreated mice (CFU-dg (CY)) and in Mo conditioned medium treated mice (CFU-dg (Mo)) were similar. On the average CFU-dm sedimented somewhat slower than CFU-dg. These and other observations suggesting a close relationship between CFU-dg and multipotential haemopoietic precursors are discussed. |
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