Sequencing and Amplified Restriction Fragment Length Polymorphism Analysis of Ribonucleotide Reductase Large Subunit Gene of the White Spot Syndrome Virus in Blue Crab (Callinectes sapidus) from American Coastal Waters |
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Authors: | Yun-Shiang Chang Shao-En Peng Han-Ching Wang Hui-Chen Hsu Ching-Hui Ho Chung-Hsiung Wang Sho-Ya Wang Chu-Fang Lo Guang-Hsiung Kou |
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Institution: | (1) Department of Zoology, National Taiwan University, Taipei, Taiwan, R.O.C., TW;(2) School of Public Health at Chung Shan Medical and Dental College, Taichung, Taiwan, R.O.C., TW;(3) Department of Entomology, National Taiwan University, Taipei, Taiwan, R.O.C., TW;(4) Department of Biological Sciences, State University of New York at Albany, New York, U.S.A., US |
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Abstract: | In the present study, the existence of white spot syndrome virus (WSSV) in blue crab (Callinectes sapidus) collected from 3 different American coastal waters (New York, New Jersey, and Texas) was confirmed by 2-step diagnostic
polymerase chain reaction and in situ hybridization analysis. When geographic isolates were also compared using a gene that
encodes the WSSV ribonucleotide reductase large subunit RR1 (WSSV rr1), a C1661-to-T point mutation was found in the New Jersey WSSV isolated. This point mutation, which resulted in the creation of an
additional RsaI endonuclease recognition site, was not found in the WSSV from the New York and Texas blue crab samples, or in the WSSV Taiwan
isolate, or in any of the other WSSV geographical isolates for which data are available. WSSV rr1-specific RsaI amplified restriction fragment length polymorphism of an amplified 1156-bp fragment thus distinguished the New Jersey blue
crab samples from the other WSSV isolates.
Received June 29, 2000; accepted October 11, 2000 |
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Keywords: | : white spot syndrome virus (WSSV) WSSV isolate Callinectes sapidus amplified restriction fragment length polymorphism ribonucleotide reductase large subunit RR1 WSSV rr1 |
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