Experimental Infection of Macaques with a Wild Water Bird-Derived Highly Pathogenic Avian Influenza Virus (H5N1) |
| |
| Authors: | Tomoko Fujiyuki Misako Yoneda Fumihiko Yasui Takeshi Kuraishi Shosaku Hattori Hyun-jeong Kwon Keisuke Munekata Yuri Kiso Hiroshi Kida Michinori Kohara Chieko Kai |
| |
| Institution: | 1. Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.; 2. Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.; 3. Amami Laboratory of Injurious Animals, The Institute of Medical Science, The University of Tokyo, Kagoshima, Japan.; 4. Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan .; Institut Pasteur, France, |
| |
| Abstract: | Highly pathogenic avian influenza virus (HPAIV) continues to threaten human health. Non-human primate infection models of human influenza are desired. To establish an animal infection model with more natural transmission and to determine the pathogenicity of HPAIV isolated from a wild water bird in primates, we administered a Japanese isolate of HPAIV (A/whooper swan/Hokkaido/1/2008, H5N1 clade 2.3.2.1) to rhesus and cynomolgus monkeys, in droplet form, via the intratracheal route. Infection of the lower and upper respiratory tracts and viral shedding were observed in both macaques. Inoculation of rhesus monkeys with higher doses of the isolate resulted in stronger clinical symptoms of influenza. Our results demonstrate that HPAIV isolated from a water bird in Japan is pathogenic in monkeys by experimental inoculation, and provide a new method for HPAIV infection of non-human primate hosts, a good animal model for investigation of HPAIV pathogenicity. |
| |
| Keywords: | |
|
|
