In Vivo Characterization of Dynein-Driven nanovectors Using Drosophila Oocytes |
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Authors: | Nadège Parassol Céline Bienvenu Cécile Boglio Sébastien Fiorucci Delphine Cerezo Xiao-Min Yu Guilhem Godeau Jacques Greiner Pierre Vierling Stéphane Noselli Christophe Di Giorgio Véronique Van De Bor |
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Institution: | 1. University of Nice Sophia-Antipolis, Institute of Biology Valrose (iBV), UMR 7277-CNRS, UMR 1091 INSERM, Nice, France.; 2. Université de Nice Sophia-Antipolis, Institut de Chimie de Nice (ICN), UMR 7272-CNRS, Nice, France.; University of Bern, Switzerland, |
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Abstract: | Molecular motors transport various cargoes including vesicles, proteins and mRNAs, to distinct intracellular compartments. A significant challenge in the field of nanotechnology is to improve drug nuclear delivery by engineering nanocarriers transported by cytoskeletal motors. However, suitable in vivo models to assay transport and delivery efficiency remain very limited. Here, we develop a fast and genetically tractable assay to test the efficiency and dynamics of fluospheres (FS) using microinjection into Drosophila oocytes coupled with time-lapse microscopy. We designed dynein motor driven FS using a collection of dynein light chain 8 (LC8) peptide binding motifs as molecular linkers and characterized in real time the efficiency of the FS movement according to its linker’s sequence. Results show that the conserved LC8 binding motif allows fast perinuclear nanoparticle''s accumulation in a microtubule and dynein dependent mechanism. These data reveal the Drosophila oocyte as a new valuable tool for the design of motor driven nanovectors. |
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