首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Expression Profile of CYP1A1 and CYP1B1 Enzymes in Colon and Bladder Tumors
Authors:Vasilis P Androutsopoulos  Ioannis Spyrou  Achilles Ploumidis  Alexandros Eystathios Papalampros  Michalis Kyriakakis  Demetrios Delakas  Demetrios A Spandidos  Aristidis M Tsatsakis
Institution:1. Laboratory of Toxicology, Department of Morphology, Faculty of Medicine, University of Crete, Heraklion, Crete, Greece.; 2. First Department of Surgery, University of Athens, Laiko Hospital, Athens, Greece.; 3. Department of Urology, “Asklipeio” General Hospital, Voula, Athens, Greece.; 4. Laboratory of Clinical Virology, Department of Laboratory Medicine, Faculty of Medicine, University of Crete, Heraklion, Crete, Greece.; Univ of Bradford, United Kingdom,
Abstract:

Background

The cytochrome P450 CYP1A1 and CYP1B1 enzymes are involved in carcinogenesis via activation of pro-carcinogenic compounds to carcinogenic metabolites. CYP1A1 and CYP1B1 have shown elevated levels in human tumors as determined by qRT-PCR and immunohistochemical studies. However studies that have examined CYP1 expression by enzyme activity assays are limited.

Results

In the current study the expression of CYP1A1 and CYP1B1 was investigated in a panel of human tumors of bladder and colorectal origin by qRT-PCR and enzyme activity assays. The results demonstrated that 35% (7/20) of bladder tumors and 35% (7/20) of colon tumors overexpressed active CYP1 enzymes. CYP1B1 mRNA was overexpressed in 65% and 60% of bladder and colon tumors respectively, whereas CYP1A1 was overexpressed in 65% and 80% of bladder and colon tumors. Mean mRNA levels of CYP1B1 and CYP1A1 along with mean CYP1 activity were higher in bladder and colon tumors compared to normal tissues (p<0.05). Statistical analysis revealed CYP1 expression levels to be independent of TNM status. Moreover, incubation of tumor microsomal protein in 4 bladder and 3 colon samples with a CYP1B1 specific antibody revealed a large reduction (72.5 ± 5.5 % for bladder and 71.8 ± 7.2% for colon) in catalytic activity, indicating that the activity was mainly attributed to CYP1B1 expression.

Conclusions

The study reveals active CYP1 overexpression in human tumors and uncovers the potential use of CYP1 enzymes and mainly CYP1B1 as targets for cancer therapy.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号