Synthesis,X-ray crystal structure,DNA/BSA binding,DNA cleavage and cytotoxicity studies of phenanthroline based copper(II)/zinc(II) complexes |
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| Authors: | Qingming Wang Han Mao Wenling Wang Huimin Zhu Lihui Dai Yanli Chen Xinhui Tang |
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| Affiliation: | 1.School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection,Yancheng Teachers’ University,Yancheng,People’s Republic of China;2.College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing,People’s Republic of China |
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| Abstract: | Research on copperII 1,10-phenanththroline (phen) derivatives continues to attract interest in the context of structure and biological properties. In this paper, two metal complexes [Cu2(phen)2(μ-Cl)2]Cl2 (1), [Zn(phen)2(H2O)Cl]Cl·4H2O (2) were synthesized and characterized. The crystal structures of 1 and 2 were determined by X-ray diffraction. In order to investigate the biological properties of the prepared complexes, spectroscopic and biological studies were performed. Results of X-ray diffraction showed that 1 and 2 form two types of crystal structures in a given system: dinuclear and mono-nuclear complex. The preliminary study on the DNA cleavage activity has shown that 1 under study behaved as the chemical nucleases. The DNA binding interaction of 1 & 2 with CT-DNA has been investigated by UV–Visible and fluorescence emission spectrometry and the apparent binding constant (K app) values are 5.1 × 104 and 1.2 × 104 M?1, respectively. In addition, fluorescence spectrometry of bovine serum albumin (BSA) with 1 & 2 showed that the quenching mechanism might be a static quenching procedure with one binding sites for BSA. In addition, the cytotoxicity of 1 in vitro on tumor cells lines (MCF-7, HepG2 and HT29) was examined by MTT and showed better antitumor effect on the tested cells. |
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