Iron modulates the activity of monoamine oxidase B in SH-SY5Y cells |
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| Authors: | Huiru Lu Jun Chen Hui Huang Mengxue Zhou Qing Zhu Shao Q Yao Zhifang Chai Yi Hu |
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| Institution: | 1.CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Multidisciplinary Research Division, Institute of High Energy Physics,Chinese Academy of Sciences (CAS),Beijing,China;2.University of Chinese Academy of Sciences,Beijing,China;3.Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering,Zhejiang University of Technology,Hangzhou,China;4.Department of Chemistry,National University of Singapore,Singapore,Singapore |
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| Abstract: | Both monoamine oxidase B (MAO-B) and iron accumulation are associated with neurologic diseases including Parkinson’s disease. However, the association of iron with MAO-B activity was poorly understood. Here we took advantage of highly sensitive and specific fluorescence probes to examine the change in MAO-B activity in human dopaminergic neuroblastoma (SH-SY5Y) cells upon iron exposure. Both ferric and ferrous ions could significantly enhance the activity of MAO-B, instead of MAO-A, in SH-SY5Y cells. In addition, iron-induced increase in MAO-B probe fluorescence could be prevented by pargyline and other newly developed MAO-B inhibitors, suggesting that it was MAO-B activity-dependent. These findings may suggest MAO-B is an important sensor in iron-stressed neuronal cells. |
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