| SARS-CoV S蛋白基因的克隆及其与VSV-G融合表达载体的构建 |
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| 引用本文： | 冯彦斌,善亚君,苑晓玲,杨振,赵振虎,王升启,刘晓辉,从玉文. SARS-CoV S蛋白基因的克隆及其与VSV-G融合表达载体的构建[J]. 生物技术通讯, 2004, 15(1): 9-12 |
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| 作者姓名： | 冯彦斌 善亚君 苑晓玲 杨振 赵振虎 王升启 刘晓辉 从玉文 |
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| 作者单位： | 1. 山西大学,生命科学与技术学院,太原,030006
2. 军事医学科学院,放射医学研究所,北京,100850 |
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| 摘 要： | 为了解重症急性呼吸综合征冠状病毒(SARS—CoV)表面S蛋白的受体结合功能域及其在宿主细胞上的作用受体，应用PCR技术从SARS—CoV cDNA中克隆到S蛋白的全长基因，并构建了S蛋白与疱疹性口腔炎病毒胞膜蛋白(VSV—G)融合表达载体pVSV—G‘-SG，进而为制备含有SARS—CoVS蛋白膜外区的逆转录病毒假毒粒奠定了实验基础。
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| 关 键 词： | SARS—COVS蛋白基因 克隆 VSV—G融合表达载体 构建 急性呼吸综合征冠状病毒 SARS |
| 文章编号： | 1009-0002(2004)01-0009-04 |
Cloning of the gene of SARS-CoV S protein and construction of the fusion expression vector of S protein with VSV-G |
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| FENG Yan-bin ,SHAN Ya-jun ,YUAN Xiao-ling ,YANG Zhen ,ZHAO Zhen-hu ,WANG Sheng-qi ,LIU Xiao-hui ,CONG Yu-wen .College of Life Science and Technology,Shanxi University,Taiyuan. Cloning of the gene of SARS-CoV S protein and construction of the fusion expression vector of S protein with VSV-G[J]. Letters in Biotechnology, 2004, 15(1): 9-12 |
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| Authors: | FENG Yan-bin SHAN Ya-jun YUAN Xiao-ling YANG Zhen ZHAO Zhen-hu WANG Sheng-qi LIU Xiao-hui CONG Yu-wen .College of Life Science Technology Shanxi University Taiyuan |
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| Affiliation: | FENG Yan-bin 1,SHAN Ya-jun 2,YUAN Xiao-ling 2,YANG Zhen 2,ZHAO Zhen-hu 2,WANG Sheng-qi 2,LIU Xiao-hui 1,CONG Yu-wen 2 1.College of Life Science and Technology,Shanxi University,Taiyuan030006,2.Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing1000850,China |
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| Abstract: | The tropism or host range could be altered by changing the viral envelope protein.In order to find the recep-tors in the host cells and invest the receptor binding domain of the severe acute respiratory syndrome coronavirus(SARS-CoV)spiking(S)protein,the full gene of S protein was cloned from the complete cDNA of SARS-CoV,and the fusion ex-pression vector pVSV-G'-SG was constructed with the extracellular domain of S protein and transmembrane region and cy-toplasmic tail of vesicular stomatitis virus(VSV)G glycoprotein by PCR method.This work is the first step to generate pseudotyped virus with S/VSV-G chimeric envelope. |
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| Keywords: | SARS-CoV S protein VSV-G retroviral expression vector |
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