Biosensor studies of the binding of extracellular matrix components with immobilized Escherichia coli O157:H7 and inhibition by polysulfated polysaccharides |
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Authors: | Marjorie B Medina |
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Institution: | (1) U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA |
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Abstract: | Binding interactions of immobilized E. coli O157:H7 with collagen I, fibronectin, laminin and glucoaminoglycans were studied utilizing a surface plasmon resonance biosensor. A model system was developed to evaluate the inhibition of collagen-laminin binding on the E. coli sensor surface with polysulfated polysaccharides such as heparan sulfate and carrageenans. Results showed that carrageenans inhibited 71–99% while heparan sulfate inhibited 39–41% of collagen/laminin binding to E. coli sensor surface. These studies allowed a rapid assessment of compounds for carcass treatment to inhibit or detach pathogens from meat and poultry. |
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Keywords: | collagen ECM E coli O157:H7 polysulfated polysaccharides SPR biosensor |
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