Recovery of nanogram quantities of DNA from plasma and quantitative measurement using labeling by nick translation |
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| Authors: | G J Fournié M Gayral-Taminh J P Bouché J J Conté |
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| Affiliation: | 1. Laboratoire d''Immunopathologie Rénale et d''Immunopharmacologie, INSERM, U 269, C.H.U. Toulouse-Purpan, 31059 Toulouse-Cédex, France;2. Centre de Recherche de Biochimie et de Génétique Cellulaire du CNRS, 118 Route de Narbonne, 31077 Toulouse-Cédex, France;1. Department of Chemistry, Amirkabir University of Technology, Tehran, Iran;2. Department of Enviromental Science & Analatical Chemistry, Stockholm University, SE10691, Stockholm, Sweden;1. Département de radiothérapie, centre Eugène-Marquis, avenue de la Bataille-Flandre-Dunkerque, 35000 Rennes, France;2. Département d’oncologie médicale, institut Curie, 25, rue d’Ulm, 75005 Paris, France;3. Université de Versailles-Saint-Quentin, ComUE, université Paris Saclay, route de l’Orme-aux-Merisiers-RD 128, 91190 Saint-Aubin, France;4. Service d’hépato-gastroentérologie, groupe hospitalier Pitié-Salpêtrière, 47-83, boulevard de l’Hôpital, 75013 Paris, France;1. Department of Medical Oncology, City of Hope, Duarte, CA;2. Division of Medical Oncology, University of Colorado Anschutz Medical Campus, Aurora, CO;3. Division of Medical Oncology, University of Washington, Seattle Cancer Care Alliance, Seattle, WA;4. Guardant Health, Redwood City, CA;1. Faculty of Physics, Babeș-Bolyai University, 400084 Cluj-Napoca, Romania;2. Faculty of Chemistry and Chemical Engineering, Babeș-Bolyai University, 400028 Cluj-Napoca, Romania;3. Clinical Institute of Urology and Renal Transplant, 400006 Cluj-Napoca, Romania;4. Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania;5. Biomed Data Analytics SRL, 400696 Cluj-Napoca, Romania;6. Department of Computer Science, Faculty of Mathematics and Computer Science, Babeș-Bolyai University, 400084 Cluj-Napoca, Romania;7. Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania;8. BIODIATECH Research Centre for Applied Biotechnology, SC Proplanta, 400478 Cluj-Napoca, Romania;9. Department of Surgical and Gynecological Oncology, Ion Chiricuta Clinical Cancer Center, 400015 Cluj-Napoca, Romania |
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| Abstract: | A method which allows for the quantitative measurement of DNA in plasma is described. After treatment of plasma with phenol, DNA is precipitated by ethanol using gelatin as a coprecipitating agent. DNA is then measured by nick translation labeling. This assay takes a few hours. It is suitable for the measurement of DNA within a range of 0.02 to 20 ng in 10 microliters of plasma. For example, it is applied to the measurement of DNA in plasma from mice injected with bacterial lipopolysaccharide. |
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