A binding assay for 25-hydroxycalciferols and 24R,25-dihydroxycalciferols using bovine plasma globulin |
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Authors: | B.W. Hollis J.H. Burton H.H. Draper |
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Affiliation: | Department of Nutrition, College of Biological Science University of Guelph Guelph, Ontario, Canada N1G 2W1 |
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Abstract: | The concentrations of the 25-hydroxy and 24R,25-dihydroxy derivatives of vitamin D were determined in 100 μ1 plasma samples using calciferol binding globulin from bovine plasma. Sufficient quantities of 24R,25-dihydroxy vitamin D were found in bovine, porcine, chicken and human plasma to interfere in the assay of 25-hydroxy vitamin D in unfractionated extracts. No metabolites of vitamin D could be found in rainbow trout plasma. |
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Keywords: | 25-(OH)D 25-hydroxycalciferol |
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