假单胞菌诱导筛选菌株PhA苯酚降解动力学及SDS对其影响

为提高苯酚降解速率，由假单胞菌(Pseudonomonas．sp)诱导筛选得到了一株能以苯酚为唯一碳源生长的新菌株Pha，并使其苯酚选择压力从400mg／L逐步提高到了700mg／L。且Pha菌株降解苯酚过程符合一级反应动力学方程。使用十二烷基磺酸钠(SDS)作为增溶剂来促进降解时，发现在SDS浓度为50～150mg／L时，降解苯酚的速率随SDS浓度增加而提高。SDS在低浓度时对其生长影响很小，但浓度达到300mg／L时，对其生长开始有了明显的抑制作用。结果标明PhA菌株有着较高的苯酚耐受浓度，SDS可以显著的提高苯酚的降解速率。SDS的理论最佳投放量为150mg／L。

Filtration of High- Concentration- Phenol - Degrading Strain PhA and Effect of SDS on its Phenol Degradation

The Pha strain was domesticated from Pseudonomonas.sp Strain AB93188.The isolated Strain grows on the medium adding phenol as sole carbon source.The pressure applied for seclection of the expected strain is concentration of phenol with the rangs between 400mg/L and 700mg/L.Kinetic Analysis for Biodegradation were discussed in this paper.Added in phenol,The experiment result indicatied that the addition of SDS can speed up the velocity of phenol degradation.While,if the concentration of SDS reaches 300mg/L,SDS began to restrain the phenol degradation velocity.The outcome indicated that 150mg/L was the best academic concentration.