The high-affinity phosphodiesterase PcPdeH is involved in the polarized growth and pathogenicity of Phytophthora capsici |
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Affiliation: | 1. Longping Branch, Graduate College, Hunan University, Changsha, 410125, China;2. Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, 410125, China;3. College of Plant Health and Medicine, The Key Laboratory of Integrated Crop Pest Management of Shandong Province, Qingdao Agricultural University, Qingdao, 266109, China;4. Shenyang Agricultural University, Plant Protection College, Shenyang, 110866, China |
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Abstract: | The cAMP signaling pathway has been shown to be important in controlling morphological changes and pathogenicity in plant pathogens. In the present study, we identified PcPdeH, a gene encoding a high-affinity phosphodiesterase (PDE), which is a key regulator of the cAMP signaling pathway. To elucidate the function of PcPdeH, PcPdeH-knockout mutants were obtained using a type II CRISPR/Cas9 system in Phytophthora capsici. The knockout transformants of PcPdeH showed vegetative growth defects and abnormal cyst germination. Infection assays indicated that compared with the wild type, PcPdeH-knockout mutants showed significantly reduced virulence on pepper and tobacco leaves and exhibited increased (1.5-2-fold) cAMP levels relative to the wild-type and CK strains. Based on these phenotypic features, we propose that PcPdeH is crucial for vegetative growth, cyst germination and pathogenicity in P. capsici. |
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Keywords: | cAMP phosphodiesterases Cyst germination Oomycete Virulence |
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