Distribution,Molecular Structure and Functional Analysis of Carnitine
Transporter (SLC22A5) in Canine Lens Epithelial Cells |
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| Authors: | Hideharu OCHIAI Nobuyuki KANEMAKI Reiichiro SATO Ken ONDA |
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| Affiliation: | 1)Research Institute of Biosciences, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan;2)Animal Teaching Hospital, Azabu University, Japan;3)Laboratory of Internal Medicine 3, Azabu University, School of Veterinary Medicine, Japan |
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| Abstract: | While carnitine has been reported to have an anti-oxidative role on the ocular surface,there has been no report on the existence of a carnitine transporter (SLC22A5) in thelens. Therefore, we investigated the carnitine transport activity of canine lensepithelial cells (LEC) and determined the molecular structure of canineSLC22A5. The carnitine transport activity was 7.16 ± 0.48 pmol/mgprotein/30 min. Butyrobetaine, the analogue of carnitine, reduced 30% of the activity at50 µM. A coding sequence of canine carnitine transporter was 1694 bplong and was predicted to encode 557 amino acid polypeptides. The deduced amino acidsequence of canine carnitine transporter showed >80% similarity to that of mouse andhuman. Western blot analysis detected the band at 60 kDa in the membrane of lensepithelial cells. The high content of carnitine in the lens is possibly transported fromaqueous humor by SLC22A5. |
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| Keywords: | carnitine cataract lens epithelial cells oxidative stress SLC22A5 |
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