Exogenous l-ascorbic acid regulates the antioxidant system to increase the regeneration of damaged mycelia and induce the development of fruiting bodies in Hypsizygus marmoreus |
| |
| Institution: | 1. National Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, No. 1000, Jinqi Road, Fengxian District, Shanghai 201403, China;2. College of Life Science, Nanjing Agricultural University, No.1, Weigang road, XuanWu District, Nanjing 210095, China;1. Pasteur Cenci Bolognetti Foundation c/o Department of Biology and Biotechnology “Charles Darwin”, Sapienza University, 00185 Rome, Italy;2. Laboratory of Functional Genomics and Protein Engineering, Biochemistry and Molecular Biology Unit, Department of Life Sciences, University of Parma, 43124 Parma, Italy;3. Institute of Biology and Molecular Pathology, CNR, 00185 Roma, Italy;4. UMR1136 INRA/Université de Lorraine “Interactions Arbres/Micro-organismes”, Lab of Excellence ARBRE, Centre INRA de Nancy, 54280 Champenoux, France;1. Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), 14965/161, Tehran, Iran;2. Department of Laboratory Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran;3. Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran;4. Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran;1. Laboratory, Biological Business Department, Ichimasa Kamaboko Co Ltd., 77-1 Junishin, Agano-shi, Niigata, 959-1936, Japan;2. Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori, 680-8553, Japan;1. Departamento de Microbiologia, Universidade Federal de Minas Gerais, MG, Brazil;2. Instituto de Geociências, Universidade Federal Fluminense, RJ, Brazil;3. Instituto Antártico Argentino, Buenos Aires, Argentina;4. Departamento de Geografia, Universidade do Estado do Rio de Janeiro, RJ, Brazil;5. Instituto de Física, Universidade Federal Fluminense, RJ, Brazil;6. Instituto René Rachou, FIOCRUZ-Minas, MG, Brazil |
| |
| Abstract: | Hypsizygus marmoreus is an important commercial edible fungus, but the lack of basic studies on this fungus has hindered further development of its commercial value. In this study, we found that the treatment of damaged vegetative mycelia with 1 mM l-ascorbic acid (ASA) significantly increased the antioxidant enzyme activities (GPX, GR, CAT and SOD) and antioxidant contents (GSH and ASA) and reduced the ROS levels (H2O2 and O2-) in mechanically damaged mycelia. Additionally, this treatment increased mycelial biomass. At the reproductive stage, our results demonstrated that the treatment of damaged H. marmoreus mycelia with 2.24 mM ASA significantly increased the antioxidant enzyme activities (GPX, GR, GST, TRXR and CAT), endogenous ASA contents and GSH/GSSG ratios in different developmental stages and significantly decreased the MDA and H2O2 contents. Furthermore, this study showed that the expression levels of the antioxidant enzyme genes were consistent with the enzyme activities. Damaged mycelia treated with ASA regenerated 2–3 d earlier than the control group and showed significantly enhanced fruiting body production. These results suggested that exogenous ASA regulated mycelia intracellular ASA content to increase mycelial antioxidant abilities, induce the regeneration of damaged mycelia and regulate the development of fruiting bodies in H. marmoreus. |
| |
| Keywords: | Antioxidant ability Fruiting body Gene expression levels Mechanically damage Mycelial growth |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
