The mitochondrial genome contribution to the phylogeny and identification of Metarhizium species and strains |
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| Affiliation: | 1. Department of Genetics and Biotechnology, Faculty of Biology, National and Kapodistrian University of Athens, Panepistimiopolis, 15701, Athens, Greece;2. Department of Biosciences, College of Science, Swansea University, Singleton Park, Swansea, SA2 8PP, Wales, United Kingdom;1. Department of Biological Sciences, Brock University, St. Catharines, ON, L2S 3A1, Canada;2. Federal Rural University of Rio de Janeiro, Brazil;1. Corporación Colombiana de Investigación Agropecuaria - Corpoica, Centro de Investigación Tibaitatá, kilómetro 14 vía Mosquera-Bogotá, Cundinamarca, Colombia;2. AgResearch, Forage Science, Lincoln Research Centre, Private Bag 4749, Christchurch 8140, New Zealand;1. Department of Entomology and Acarology, “Luiz de Queiroz” College of Agriculture (ESALQ/USP), Av. Pádua Dias 11, 13418-900, Piracicaba, SP, Brazil;2. Department of Genetics, “Luiz de Queiroz” College of Agriculture (ESALQ/USP), Av. Pádua Dias 11, 13418-900, Piracicaba, SP, Brazil;3. São Paulo''s Agency for Agribusiness Technology (APTA), Rodovia SP 127, Vila Fatima, 13400-970, Piracicaba, SP, Brazil;1. Department of Biology and Chemistry, Bozhou College, Bozhou 236800, China;2. Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, China;3. College of Landscape and Art, Jiangxi Agricultural University, Nanchang 330045, China;1. Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás (UFG), Goiânia 74605-050, Goiás, Brazil;2. Centro Universitário de Gurupí (UnirG), Gurupí 77425-500, Tocantins, Brazil;3. Instituto Federal de Educação, Ciência e Tecnologia de Goiás (IFG), Aparecida de Goiânia 74968-755, Goiás, Brazil;4. Embrapa Recursos Genéticos e Biotecnologia, Brasília 70770-917, Distrito Federal, Brazil;5. USDA-ARS Emerging Pests and Pathogens Research Unit, Robert W. Holley Center for Agriculture and Health, Ithaca, NY, 14853-2901, USA |
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| Abstract: | The genus Metarhizium is composed of entomopathogenic fungal biological control agents (BCAs) used for invertebrate pest control. The phylogenetic relationships of species within this genus are still under scrutiny as several cryptic species can be found. In this work, the mitochondrial (mt) genome of Metarhizium brunneum ARSEF 4556 was fully sequenced and a comparative genome analysis was conducted with 7 other available mt genomes, belonging to 5 Metarhizium species: M. anisopliae, M. brunneum, M. robertsii, M. guizhouense and M. majus. Results showed that Metarhizium demonstrates greater conserved stability than other fungal mt genomes. Furthermore, this analysis located 7 diverse regions in both intergenic domains and gene fragments which were ideal for species/strain discrimination. The sequencing of these regions revealed several SNPs among 38 strains tested, 11 of which were uncharacterized. Single gene phylogenies presented variable results which may be used further for intra-species discrimination. Phylogenetic trees based on the concatenation of mt domains and the nuclear ITS1-5.8S-ITS2 region showed discrimination of the species studied and allowed the identification of uncharacterized strains. These were mostly placed within species M. anisopliae and M. brunneum. Five strains clustered together in a clade related to M. brunneum, suggesting that they comprise a cryptic species. |
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| Keywords: | Mitochondrion Entomopathogenic fungi Cryptic species Phylogenetic relationships Species identification Strain identification |
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