Production of ethanol and xylanolytic enzymes by yeasts inhabiting rotting wood isolated in sugarcane bagasse hydrolysate |
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| Institution: | 1. Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil;2. Programa de Pós-Graduação em Botânica, Laboratório de Micologia, Departamento de Botânica, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brazil;3. Departamento de Ciências Biológicas e Agroindústria, Universidade Estadual Santa Cruz, Ilhéus, BA 45662-900, Brazil;4. Department of Biology, University of Western Ontario, London, ON N6A 5B7, Canada;1. Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, 58038, Morelia, Mich, Mexico;2. Laboratorio Estatal de Salud Pública del Estado de Michoacán, 58279, Morelia, Mich, Mexico;3. Departamento de Química, Universidad de Guanajuato, 36000, Guanajuato, Gto., Mexico;4. Departamento de Ciencias Médicas, Universidad de Guanajuato, 37320, León, Gto, Mexico;5. Departamento de Genética y Microbiología, Universidad de Murcia, 30100 Murcia, Spain;1. Department of Clinical Laboratory, the Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510700, China;2. Department of Clinical Laboratory, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong 519000, China;3. Department of Clinical Laboratory, Guangzhou No. 8 People''s Hospital, Guangzhou, Guangdong 510000, China;4. Department of Clinical Laboratory, Guangzhou No. 12 People''s Hospital, Guangzhou, Guangdong 510620, China;5. Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, China;6. Department of Clinical Laboratory, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, China;1. Laboratory of Environmental Microbiology, Institute of Microbiology of the CAS, Vídeňská 1083, 14220 Praha 4, Czech Republic;2. Department of Genetics and Microbiology, Faculty of Science, Charles University, Vini?ná 5, 12843 Prague 2, Czech Republic;3. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Brunswick, Inhoffenstrasse 7B, 38124 Braunschweig, Germany;1. School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Avenida Brasil 2085, Valparaíso, Chile;2. Green Technology Research Group, Facultad de Ingeniería y Ciencias Aplicadas, Universidad de los Andes, Mons. Álvaro del Portillo 12455, Las Condes, Santiago 7620001, Chile;3. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kongens Lyngby, Denmark |
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| Abstract: | Yeasts associated with rotting wood from four Atlantic Rain forest sites in Brazil were investigated using a culture medium based on sugarcane bagasse hydrolysate. A total of 330 yeast strains were isolated. Pichia manshurica, Candida pseudolambica, and Wickerhamomyces sp. 3 were the most frequently isolated species. Fourteen novel species were obtained in this study. All isolates were tested for their ability to ferment d-xylose and to produce xylanases. In the fermentation assays using d-xylose (30 g L?1), the main ethanol producers were Scheffersomyces stipitis (14.08 g L?1), Scheffersomyces sp. (7.94 g L?1) and Spathaspora boniae (7.16 g L?1). Sc. stipitis showed the highest ethanol yield (0.42 g g?1) and the highest productivity (0.39 g L?1h?1). The fermentation results using hemicellulosic hydrolysate showed that Sc. stipitis was the best ethanol producer, achieving a yield of 0.32 g g?1, while Sp. boniae and Scheffersomyces sp. were excellent xylitol producers. The best xylanase-producing yeasts at 50 °C belonged to the species Su. xylanicola (0.487 U mg?1) and Saitozyma podzolica (0.384 U mg?1). The results showed that rotting wood collected from the Atlantic Rainforest is a valuable source of yeasts able to grow in sugarcane bagasse hydrolysate, including species with promising biotechnological properties. |
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| Keywords: | Atlantic rainforest Scheffersomyces Spathaspora Xylanase prodution Xylose fermentation |
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