Colonization of Metarhizium anisopliae on the surface of pine tree logs: A promising biocontrol strategy for the Japanese pine sawyer,Monochamus alternatus |
| |
Affiliation: | 3. Futura Gene Biotechnology (Shanghai) Co., Ltd., 200233, Shanghai, China;1. Department of Agricultural Biology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju 561-756, Korea;2. Department of Biotechnology and Animal Science, National Ilan University, Ilan, Taiwan;3. Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul, Korea |
| |
Abstract: | We investigated the colonization potential of five Metarhizium anisopliae isolates on pine tree surfaces under laboratory conditions, determined the influence of the pine bark extract on fungal growth and evaluated the insecticidal activity following colonization on the Japanese pine sawyer. Finally, the effect of colonization on adults pine sawyer was evaluated using the top three performing isolates (JEF-197, JEF-271 and JEF-279) under laboratory and field conditions. As a result, isolate JEF-197 showed the highest conidial production on the pine surfaces, and five isolates, including JEF-197, showed higher hyphal growth on autoclaved pine bark extract agar, compared to a water agar. Pine bark treated with the isolates showed 40–70 % mortality of adults pine sawyer. Under mimicked overwintering conditions, in the JEF-197 treatment group, 40 % of the inserted larvae became adults and all were dead after 59 d. In a field test, colonized isolate JEF-197 also showed 37 % insecticidal activity against emerged adults from the pine logs as overwintering sites. This work suggests that M. anisopliae isolate JEF-197 possibly colonized the pine surface and application of a conidial suspension on the pine logs as overwintering sites could be an effective strategy to control the pine sawyer. |
| |
Keywords: | Biopesticides Entomopathogenic fungi Forest pest control Fungal colonization Pine logs |
本文献已被 ScienceDirect 等数据库收录! |
|