The chaperone Lhs1 contributes to the virulence of the fish-pathogenic oomycete Aphanomyces invadans |
| |
| Institution: | 1. Aberdeen Oomycete Laboratory, Institute of Medical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, Scotland, United Kingdom;2. International Centre for Aquaculture Research and Development (ICARD), University of Aberdeen, Scotland, United Kingdom;3. Faculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, 21030, Malaysia;4. Fish Health Management Division, National Bureau of Fish Genetic Resources, 226002, Lucknow, India;5. Molecular Biology I, Centre for Medical Biotechnology, University of Duisburg-Essen, 45141, Essen, Germany;1. School of Biology, Newcastle University, Newcastle upon Tyne NE1 7RU, UK;2. Department of Biological Sciences, Northern Illinois University, Dekalb, IL 60115, USA;3. Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, East Sussex BN1 9RQ, UK;4. Department of Ecology and Evolutionary Biology, University of Michigan, Kraus Natural Science Bld., Rm. 1008, 830 North University, Ann Arbor, MI 48109, USA;1. Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, the Netherlands;2. Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK;1. ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226002, Uttar Pradesh, India;2. Peninsular and Marine Fish Genetic Resources Centre, ICAR-NBFGR, CMFRI Campus, Kochi, 682 018, Kerala, India;1. Aberdeen Oomycete Laboratory, University of Aberdeen, School of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK;2. School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ, UK |
| |
| Abstract: | Oomycetes are fungal-like eukaryotes and many of them are pathogens that threaten natural ecosystems and cause huge financial losses for the aqua- and agriculture industry. Amongst them, Aphanomyces invadans causes Epizootic Ulcerative Syndrome (EUS) in fish which can be responsible for up to 100% mortality in aquaculture. As other eukaryotic pathogens, in order to establish and promote an infection, A. invadans secretes proteins, which are predicted to overcome host defence mechanisms and interfere with other processes inside the host. We investigated the role of Lhs1 which is part of an ER-resident complex that generally promotes the translocation of proteins from the cytoplasm into the ER for further processing and secretion. Interestingly, proteomic studies reveal that only a subset of virulence factors are affected by the silencing of AiLhs1 in A. invadans indicating various secretion pathways for different proteins. Importantly, changes in the secretome upon silencing of AiLhs1 significantly reduces the virulence of A. invadans in the infection model Galleriamellonella. Furthermore, we show that AiLhs1 is important for the production of zoospores and their cluster formation. This renders proteins required for protein ER translocation as interesting targets for the potential development of alternative disease control strategies in agri- and aquaculture. |
| |
| Keywords: | Oomycete Epizootic ulcerative syndrome (EUS) Virulence Protein secretion Pathogen Aquaculture Effector |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
