| 载脂蛋白E3的克隆及原核表达 |
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| 引用本文： | 李会成,李玉新,金萍,张聪,乌垠,陆军,杨红,麻彤晖,黄百渠. 载脂蛋白E3的克隆及原核表达[J]. 中国生物化学与分子生物学报, 2003, 19(1): 133-135 |
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| 作者姓名： | 李会成 李玉新 金萍 张聪 乌垠 陆军 杨红 麻彤晖 黄百渠 |
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| 作者单位： | 东北师范大学细胞与遗传学研究所,长春,130024 |
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| 摘 要： | 载脂蛋白Ｅ(ａｐｏｌｉｐｏｐｒｏｔｅｉｎＥ ,ａｐｏＥ)由 2 99个氨基酸组成 ,分子量 34ｋＤ ,是维持人体正常脂质代谢的必需蛋白质 .它是乳糜微粒 (ＣＭ )、极低密度脂蛋白 (ＶＬＤＬ)和高密度脂蛋白 (ＨＤＬ)的组分 ,是极低密度脂蛋白受体的重要配体 ,是脂质进入细胞不可缺少的中介 .ａｐｏＥ有 3种同分异构体 :ａｐｏＥ2、ａｐｏＥ3、ａｐｏＥ4 ,分别具有不同的生理作用 .ａｐｏＥ4与血浆高胆固醇、心血管疾病和老年痴呆等疾病关联[1～ 3 ] .ａｐｏＥ2与Ⅲ型高脂血症有关 ,并对老年痴呆有防治作用[4,5] .ａｐｏＥ3是大多数健康人所具有…
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| 收稿时间： | 2003-02-20 |
| 修稿时间： | 2002-06-05 |
The Cloning of Apolipoprotein E3 Gene and Its Expression in E.coli |
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| LI Hui cheng,LI Yu xin,JIN Ping,ZHANG Cong,WU Yin,LU Jun,YANG Hong,MA Tong hui,HUANG Bai qu. The Cloning of Apolipoprotein E3 Gene and Its Expression in E.coli[J]. Chinese Journal of Biochemistry and Molecular Biology, 2003, 19(1): 133-135 |
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| Authors: | LI Hui cheng LI Yu xin JIN Ping ZHANG Cong WU Yin LU Jun YANG Hong MA Tong hui HUANG Bai qu |
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| Affiliation: | (Institute of Cytology and Genetics,Northeast Normal University,Chuangchun 130024,China |
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| Abstract: | The apoE cDNA was amplified from four months old fetus liver tissue mRNA by RT PCR. It was inserted into the TOPO ○R vector, whose sequence is the same as that reported. The right apoE cDNA was inserted into pQE30 vector to construct pQE30 apoE recombinant expressed plasmid, which was expressed as a histidine tag fusion protein in E.coli strain BL21(DE3) with higher level. The purified protein was gained by Ni NTA column. SDS PAGE showed that 34 kD protein was expressed. Western blot analysis showed a positive band at 34 kD. |
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| Keywords: | human apoE 3 cDNA molecular cloning gene expression |
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