Grafting of triggering site onto lymphocytes; distribution of grafted dinitrophenyl groups on cell surface glycoproteins and glycolipids |
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Authors: | Sarah Spiegel Meir Wilchek |
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Affiliation: | 1. Department of Biophysics, The Weizmann Institute of Science, P.O.B. 26, 76100, Rehovot, Israel
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Abstract: | Quantitative investigation of membrane-bound sialoglycoconjugates on lymphocyte surface was performed by chemical modification of the sialic acid residues with radioactive N4-dinitrophenyl-L-2,4-diaminobutyric acid hydrazide (DNP-DABH). This labeled both glycoproteins and glycolipids with concomitant preservation of the mitogenic activity by multivalent hapten binding protein (anti-DNP antibody). Under conditions where maximum stimulation of thymocytes occurred radioactive DNP-DABH labeled 1.1 X 10(7) glycolipids molecules/cell but, only 3 X 10(6) glycoproteins molecules/cell. When B lymphocytes, which do not undergo DNP-mediated stimulation were used, glycolipids labeling could not be detected. Major differences between stimulation committed and non-committed DNP-modified lymphocytes was the amount of ligand attached to the cell surface sialoglycolipids (gangliosides). |
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