Characterisation of the dynamic behaviour of lipid droplets in the early mouse embryo using adaptive harmonic generation microscopy |
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| Authors: | Tomoko Watanabe Anisha Thayil Alexander Jesacher Kate Grieve Delphine Debarre Tony Wilson Martin Booth Shankar Srinivas |
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| Institution: | (1) Department of Physiology Anatomy and Genetics, University of Oxford, Oxford, OX1 3QX, UK;(2) Department of Engineering Science, University of Oxford, Oxford, OX1 3PJ, UK |
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| Abstract: | Background Lipid droplets (LD) are organelles with an important role in normal metabolism and disease. The lipid content of embryos has
a major impact on viability and development. LD in Drosophila embryos and cultured cell lines have been shown to move and
fuse in a microtubule dependent manner. Due to limitations in current imaging technology, little is known about the behaviour
of LD in the mammalian embryo. Harmonic generation microscopy (HGM) allows one to image LD without the use of exogenous labels.
Adaptive optics can be used to correct aberrations that would otherwise degrade the quality and information content of images. |
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