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耐碱的偶氮染料脱色菌筛选及其特性的研究
引用本文:张胜琴,陈必强,杨光,李春,曹竹安. 耐碱的偶氮染料脱色菌筛选及其特性的研究[J]. 中国生物工程杂志, 2010, 30(5): 76-80
作者姓名:张胜琴  陈必强  杨光  李春  曹竹安
作者单位:1.石河子大学化学化工学院 石河子 832003 2.清华大学化工系生物化工研究所 北京 1000843.中国科学院天津工业技术生物研究所 天津 3003084.北京理工大学生命学院 北京 100081
基金项目:国家"863"计划
摘    要:从浙江某污水处理厂的活性污泥中筛选出若干株在高pH条件下对偶氮染料酸性大红GR有脱色能力的菌株,经脱色验证得到一株具有高效脱色活性的菌株Z1,经鉴定为巴斯德葡萄球菌(Staphylococcus pasteuri),并对此菌株的脱色特性进行了初步研究。结果表明,在厌氧条件下,Z1在pH7~12,40h对50mg/L的酸性大红GR脱色率均可达90%以上。该菌株对染料有较强的耐受力,在酸性大红GR浓度为300mg/L时,48h的脱色率仍可达93%。此外,该菌株能够对多种偶氮染料脱色,具有较好的脱色广谱性,有望应用于处理工业废水中的偶氮染料。

关 键 词:偶氮染料  巴斯德葡萄球菌  耐碱菌  广谱性  
收稿时间:2010-03-10
修稿时间:2010-04-23

Screening and Characteristics of Azo Dye Decoloring Alkali-resistant Bacteria
ZHANG Sheng-qin,CHEN Bi-qiang,YANG Guang,LI Chun,CAO Zhu-an. Screening and Characteristics of Azo Dye Decoloring Alkali-resistant Bacteria[J]. China Biotechnology, 2010, 30(5): 76-80
Authors:ZHANG Sheng-qin  CHEN Bi-qiang  YANG Guang  LI Chun  CAO Zhu-an
Affiliation:1.College of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China2.Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China3.Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China4.School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China
Abstract:Studies were carried out to isolate Acid Scarlet GR resistant and decolored bacteria from sludge of a wastewater treatment plant, Zhejiang Province. Through dyeing decoloring tests, one strain, named Z1 was found to have a better decolorization performance at the high pH 12 and identified as Staphylococcus pasteuri by 16SrDNA gene sequence analysis. A strain named Z1 was screened through dye decolorization test and was identified as Staphylococcus pasteuri by 16SrDNA gene sequence analysis. And then the decolorization properties of Z1 were investigated. The results showed that the decolorization rate of 50mg/L Acid Scarlet GR could exceed 90% within 40h under anaerobic conditions at the range of pH 7~12, and the decolorization rate of 300mg/L Acid Scarlet GR even reached 93% within 48h. Moreover, the strain could effectively degrade other azo dyes with broad spectrum and could be applied for removing the azo dyes from the industrial wastewater.
Keywords:azo dye  Staphylococcus pasteuri  Alkali-resistant bacteria  broad-spectru
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