| 大鼠肾脏组织cDNA文库的制备和葡萄糖转运蛋白cDNA克隆的筛选 |
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| 引用本文： | 程振起,许卫东,蒋玉玲,黄永芬,汪清胤. 大鼠肾脏组织cDNA文库的制备和葡萄糖转运蛋白cDNA克隆的筛选[J]. 中国生物化学与分子生物学报, 1991, 7(5): 578-582 |
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| 作者姓名： | 程振起 许卫东 蒋玉玲 黄永芬 汪清胤 |
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| 作者单位： | 清华大学生物科学与技术系，清华大学生物科学与技术系，清华大学生物科学与技术系，哈尔滨师范大学生物系，哈尔滨师范大学生物系 北京 100084 中国科学院生物物理所生物大分子国家重点实验室，北京 100084 中国科学院生物物理所生物大分子国家重点实验室，北京 100084 中国科学院生物物理所生物大分子国家重点实验室，哈尔滨 150080，哈尔滨 150080 |
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| 摘 要： | 大鼠的肾脏组织经匀浆后,提取纯化总RNA和mRNA合成cDNA,进行甲基化,加人工接头,最后连入载体(λgt11)和外壳蛋白包装步骤制成肾脏组织的cDNA文库。插入载体的cDNA长度为0.5kb到8kb之间。经反复稀释,测定出该文库的效价为1.5×10~7pfu/mL。
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| 关 键 词： | cDNA文库 cDNA克隆 mRNA纯化 分子杂交和筛选 |
| 收稿时间： | 1991-10-20 |
Preparation of Rat Kidney cDNA Library and Screening of Glucose Transporter cDNA Clone |
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| Cheng,Zhen-qi Xu,Wei-dong Jiang,Wu-ling Huang,yong-fen Wang,Qing-yin. Preparation of Rat Kidney cDNA Library and Screening of Glucose Transporter cDNA Clone[J]. Chinese Journal of Biochemistry and Molecular Biology, 1991, 7(5): 578-582 |
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| Authors: | Cheng Zhen-qi Xu Wei-dong Jiang Wu-ling Huang yong-fen Wang Qing-yin |
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| Affiliation: | (Department of biological science and biotechnology, Tsinghua university, Beijing 100084)(National Lab of Biomacromolecules, Institute of Biophysics Academia Sinica) (Department of biology, Harbin teacher university, Harbin 150080 |
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| Abstract: | Rat tissues were homogenized followed by purification of total RNAs and mRNAs, synthesizing cDNA, methylation, linking and finally ligation of the cDNA into λgt11 vector and packaging the length of the cDNA was 0.5-8kb. The titer was about 1.5×107pfu/mL.One positive cDNA clone was obtained by screening of the cDNA library using gluose transporter cDNA of rat liver as a probe. The fragments of the cDNA were obtained by digestion of the positive clone with E. coR1. |
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| Keywords: | cDNA library cDNA clone mRNA purification Molecular hybridization and screening |
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