Non-symmetrical cytosine methylation in tobacco pollen DNA |
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Authors: | Edward J Oakeley Jean-Pierre Jost |
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Institution: | (1) Friedrich Miescher-Institut, Postfach 2543, CH-4002 Basel, Switzerland |
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Abstract: | We have detected sequence-specific non-symmetrical cytosine methylation within a 140 bp region of the promoter for the tobacco auxin-binding protein gene T85 in pollen DNA. Direct sequencing of the population of bisulphite reaction products showed that, in this region, 10 out of a possible 49 cytosine residues were methylated at a high frequency in pollen whereas the corresponding region from somatic cells (leaf DNA) did not show a detectable level of methylation. The context of these sites was 1×m5CpTpC, 1×m5CpGpT, 1×m5CpCpT, 2×m5CpTpT, 2×m5CpGpG, and 3×m5CpApT of which only m5CpGpG and m5CpGpT fitted the consensus sequence for symmetrical methylation in plants. |
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Keywords: | DNA methylation auxin-binding protein genomic sequencing Nicotiana tabacum |
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