| 制备遗传工程小鼠的技术改进 |
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| 引用本文： | 相学军,陈艳秋,张新蕊,朱必才.制备遗传工程小鼠的技术改进[J].生物技术通报,2006(6):72-75,96. |
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| 作者姓名： | 相学军 陈艳秋 张新蕊 朱必才 |
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| 作者单位： | 徐州师范大学生命科学学院,徐州,221116 |
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| 基金项目： | 国家自然科学基金资助项目(NO.30470966,NO.30270738) |
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| 摘 要： | 遗传工程小鼠是当今生命科学领域集成度最高的研究体系之一。特别在“人类基因组计划和小鼠基因组计划”完成后,遗传工程小鼠在制备人类疾病模型、药物开发和评价、基因功能分析以及比较基因组学中发挥着越来越重要的作用。由此,也推动了遗传工程小鼠相关技术的快速发展。就遗传工程小鼠制备的现况、存在的问题以及新策略等相关问题进行了总结。
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| 关 键 词： | 遗传工程小鼠 靶基因 位点特异性重组酶 条件性转基因 条件性基因剔除 |
| 收稿时间： | 2006-05-26 |
| 修稿时间： | 2006-05-26 |
Technical Improvement of Preparing Genetically Engineered Mouse |
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| Xiang Xuejun,Chen Yanqiu,Zhang Xinrui,Zhu Bicai.Technical Improvement of Preparing Genetically Engineered Mouse[J].Biotechnology Bulletin,2006(6):72-75,96. |
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| Authors: | Xiang Xuejun Chen Yanqiu Zhang Xinrui Zhu Bicai |
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| Institution: | School of Life Science, Xuzhou Normal University, Xuzhou 221116 |
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| Abstract: | Today,Genetically Engineered Mouse(GEM) is one of the most powerful research systems in life science area.Especially,since the 'Human Genomic and Mouse Genomic Project' have been finished,Genetically Engineered Mouse is becoming increasingly useful in generating the animal-model of human disease,developing and evaluating new drugs,analysing the gene functions and also in comparative genomics,which make the technologies of GEM develope rapidly.In this review,we summarized the current state of producing GEM model,some questions and recent strategies,and so on. |
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| Keywords: | Genetically engineered mouse Target gene Site-specific recombinase Conditional transgene Conditional gene knock-out |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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