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Structure of equine corticotropin releasing factor |
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| Authors: | John H Livesey Alan Carne Clifford H G Irvine Jane Ellis Margaret J Evans Roger Smith and Richard A Donald |
| Institution: | * Department of Endocrinology, The Princess Margaret Hospital, Christchurch, New Zealand † Department of Biochemistry, University of Otago, Dunedin, New Zealand ‡ Veterinary and Animal Sciences Group, Lincoln University, New Zealand |
| Abstract: | A 41 amino acid peptide, probably identical in structure to human corticotropin releasing factor, was isolated from 70 equine hypothalami by methanol extraction, immunoaffinity chromatography and single step of reverse phase HPLC. The amino acid sequence was determined by gas phase sequence analysis. Probable carboxyl terminal amidation was demonstrated by similar retention times for equine and human corticotropin releasing factor on reverse phase HPLC at pH 8. The likely structure of equine corticotropin releasing factor is: Ser-Glu-Glu-Pro-Pro- Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn- Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2. The purified peptide is equipotent with human corticotropin releasing factor in an in vitro bioassay and in a human plasma binding protein assay. |
| Keywords: | Corticotropin releasing factor Neuropeptides Isolation Sequence Horse Hypothalamus |
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