| 小鼠组织相容性抗原的提取与纯化 |
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| 引用本文： | 兰平,顾熊飞,王吉甫. 小鼠组织相容性抗原的提取与纯化[J]. 中国实验动物学报, 1997, 0(1) |
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| 作者姓名： | 兰平 顾熊飞 王吉甫 |
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| 作者单位： | 华西医科大学附属一院外科(兰平)，中山医科大学生化教研室(顾熊飞)，中山医科大学附属一院普外科(王吉甫) |
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| 摘 要： | 以建立方便、大量纯化组织相容性抗原的方法为目的。用０．５％Ｔｒｉｔｏｎ／Ｔｒｉｓ抽提小鼠组织相容性抗原（Ｈ－２）抗原，利用抗Ｈ－２抗原抗体制备的亲和柱，特异性结合Ｈ－２抗原，再用０．５％ＤＯＣ、０．６５ＭＮａＣｌ洗脱结合Ｈ－２抗原。结果显示：电泳显示纯化物为４５ｋｄ（重链），１２ｋｄ（轻链）两条带，纯化物具有明显的血清学及生物学活性；这种亲和层析法可大量纯化组织相容性抗原，用于器官移植研究及组织相容性抗原的免疫功能研究。
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| 关 键 词： | 主要组织相容性抗原 去污剂 亲和层析 |
Extraction and Purification of Major Histocompatibility Complex Antigen in Mouse |
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| Lan Ping,et al. Extraction and Purification of Major Histocompatibility Complex Antigen in Mouse[J]. Acta Laboratorium Animalis Scientia Sinica, 1997, 0(1) |
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| Authors: | Lan Ping et al |
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| Abstract: | Objective:This study was designed to purify Major Histocompatibility Complex (MHC) antigen (H 2) that induce the rejection of organ transplantation in mouse.Methods:Triton/tris was used to extract the crude H 2,to which of the anti H 2 antibodies coupled with sepharose 4B was applied to purify H 2 antigen by affinity chromatography.The coupled H 2 Ag was eluted via 0.5%DOC,0.65M NaCl solution.Results:45kd heavy chain and 12kd light chain from purified proteins showed respectively by electrophoresis.Besides,the pure H 2 Ag was found to have serologically and biologically activity,as determined by antibodies rebinding and cytotoxicity inhibition assay.This method should be useful purification of a large quantity of H 2 Ag for the research in transplantation and functional analysis of MHC antigen. |
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| Keywords: | Major Histocompatibility complex Detergent Affinity chromatography |
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