Biochemical characterization of Artemia ras p21 |
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| Authors: | Alberto Gandarillas Jaime Renart Miguel Quintanilla |
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| Institution: | (1) Instituto de Investigaciones Biomédicas del CSIC, Arturo Duperier, 4, 28029 Madrid, Spain |
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| Abstract: | The biochemical properties of Artemia ras proteins (p21) have been studied after immunoprecipitation with the monoclonal antibody Y13-259. The ras products bind GTP and GDP, and have GTPase activity. Artemia p21 was unable to hydrolyze GP4G, although this dinucleotide exhibits high affinity for the protein. Our results demonstrate that the protein(s) recognized by the Y13-259 antibody in this crustacean behave as typical mammalian ras p21s. |
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| Keywords: | Artemia ras proteins nucleotide binding GTPase activity |
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