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| 干扰骨髓基质细胞Hes1基因对Mash1基因表达的影响 | |
| 引用本文： | 龙志晶,王春芳,李鹏飞,刘彩玉,邓春圣,呼钟理. 干扰骨髓基质细胞Hes1基因对Mash1基因表达的影响[J]. 中国组织化学与细胞化学杂志, 2011, 20(3): 257-261. DOI: 10.3870/zgzzhx.2011.03.011 |
| 作者姓名： | 龙志晶 王春芳 李鹏飞 刘彩玉 邓春圣 呼钟理 |
| 作者单位： | 1. 山西医科大学生物技术教研室,太原,030001 2. 中国人民解放军第322医院,山西大同,037006 3. 太原市中医医院,太原,030002 |
| 基金项目： | 山西省自然科学基金，中国人民解放军第322医院自然科学基金 |
| 摘 要： | 目的观察对体外培养的骨髓基质细胞Hes1基因干扰后Mash1基因的表达变化。方法采用全骨髓培养法体外分离培养获得骨髓基质细胞,倒置显微镜下观察其形态变化,应用实时定量PCR技术分析在干扰Hes1基因表达的情况下Mash1基因表达的变化。结果应用200nM,100nM,50nM,25nM四种不同浓度梯度的Hes1的siRNA干扰抑制Hes1基因,与对照组相比Hes1表达均降低。200nM干扰后Hes1基因降低的程度是原来的0.036(P<0.05),50nM干扰后Hes1基因降低的程度是原来的0.076(P<0.05),优于其他浓度的干扰效果。Hes1降低可使Mash1基因表达增加,且Hes1降低越显著,Mash1表达增加越高。结论体外培养BMSCs过程中干扰Hes1基因对Mash1基因的表达有影响,且呈负相关调控关系。 |
| 关 键 词： | 骨髓基质细胞 基因表达 Hes1基因 Mash1基因 RNAi |
The expression of Mash1 after the inhibition of Hes1 gene in BMSCs |
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| Long Zhijing,Wang Chunfang,Li Pengfei,Liu Caiyu,Deng Chunsheng,Hu Zhongli. The expression of Mash1 after the inhibition of Hes1 gene in BMSCs[J]. Chinese Journal of Histochemistry and Cytochemistry, 2011, 20(3): 257-261. DOI: 10.3870/zgzzhx.2011.03.011 | |
| Authors: | Long Zhijing Wang Chunfang Li Pengfei Liu Caiyu Deng Chunsheng Hu Zhongli |
| Affiliation: | Long Zhijing1,Wang Chunfang1,Li Pengfei1,Liu Caiyu1 Deng Chunsheng2,Hu Zhongli3(1Department of Biotechnology,Shanxi Medical University,Taiyuan 030001,2The 322ndHospital of PLA,Datong Shanxi 037006,3Taiyuan Hospital of Traditional Chinese Medicine,Taiyuan 030002,China) |
| Abstract: | Objective To explore the expression of Mash1 after the inhibition of the Hes1 gene in BMSCs.Methods BMSCs isolated from bone marrow were cultured in vitro.The morphologic feature and growth status of BMSCs and differentiated BMSCs were observed by inverted microscopy.The expression of Mash1 was analyzed by real-time quantitative PCR.Results The concentrations of Hes1 siRNA were 200nM,100nM,50nM or 25nM.The expression of Hes1 was decreased in comparison with that of the control group.The level of Hes1 at the... |
| Keywords: | RNAi |
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