A novel virus that infecting hypovirulent strain XG36-1 of plant fungal pathogen Sclerotinia sclerotiorum |
| |
Authors: | Liyan Zhang Yanping Fu Jiatao Xie Daohong Jiang Guoqing Li Xianhong Yi |
| |
Institution: | 1. Division of Biochemistry and Biotechnology, National Institute of Communicable Diseases (NICD), 22 Shamnath Marg, Delhi, 110054, India 2. Division of Virology, Defence Research & Development Establishment, Jhanshi Road, Gwalior, 474002, MP, India 3. Division of Zoonosis, National Institute of Communicable Diseases (NICD), 22 Shamnath Marg, Delhi, 110054, India
|
| |
Abstract: | Background Dengue virus type 1 (DENV-1) have been mostly circulating silently with dominant serotypes DENV-2 and DENV-3 in India. However recent times have marked an increase in DENV-1 circulation in yearly outbreaks. Many studies have not been carried out on this virus type, leaving a lacunae pertaining to the circulating genotypes, since its earliest report in India. In the present study, we sequenced CprM gene junction of 13 DENV-1 isolated from Delhi and Gwalior (North India) between 2001–2007 and one 1956 Vellore isolate as reference. For comparison, we retrieved 11 other Indian and 70 global reference sequences from NCBI database, making sure that Indian and global isolates from all decades are available for comparative analysis. Results The region was found to be AT rich with no insertion or deletion. Majority of the nucleotide substitutions were silent, except 3 non-conservative amino acid changes (I → T, A → T and L → S at amino acid positions 59,114 and 155 respectively) in the Indian DENV-1 sequences, sequenced in this study. Except two 1997–98 Delhi isolates, which group in genotype I; all other Indian isolates group in genotype III. All Indian genotype III DENV-1 exhibited diversity among them, giving rise to at least 4 distinct lineages (India 1–4) showing proximity to isolates from diverse geographic locations. Conclusion The extensive phylogenetic analysis revealed consistent existence of multiple lineages of DENV-1 genotype III during the last 5 decades in India. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|