The Detection of Nonhybrid, Trisomic, and Triploid Offspring in Sexual Progeny of a Mating of Phytophthora infestans |
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Authors: | Dee A Carter Kenneth W Buck Simon A Archer Theo Van der Lee Richard C Shattock David S Shaw |
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Institution: | a Department of Biology, Imperial College of Science, Technology and Medicine, London, SW7 2AZ, United Kingdom;b Department of Phytopathology and Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Binnehaven 9, 6709 PD, Wageningen, The Netherlands;c School of Biological Sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, United Kingdom |
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Abstract: | Eighty single-oospore offspring of Phytophthora infestans from a mating of isolates, which had previously been analyzed for segregation of avirulence/virulence, were assessed for the inheritance of 20 RFLP markers. Three offspring were triploid; they inherited three alleles at all loci where this could be detected and when heterozygous, showed unequal intensities of hybridization with most probes. Twenty-four offspring were trisomic, as each had three doses of one or a few markers, evident from their inheritance of three alleles or from unequal hybridization to one probe. Coinheritance of the extra allele(s) and mitochondrial haplotype in the majority of trisomic offspring suggested that meiosis in oogonia was more aberrant than in antheridia. Linkage analysis was performed on 50 offspring, which were assumed to be euploid; six small linkage groups were detected and several avirulence loci were found to be linked. The origins of aberrant offspring are discussed. |
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Keywords: | Phytophthora infestans RFLP linkage avirulence mitochondrial DNA triploidy trisomy aneuploidy Oomycetes |
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