中国松毛虫属八个种和亚种亲缘关系的DNA指纹证据

利用DNA指纹谱方法探讨了中国松毛虫属8个种和亚种〔马尾松毛虫Dendrolimus punctatus punctatus (Walker)，德昌松毛虫D. punctatus tehchangensis Tsai et Liu，文山松毛虫D. punctatus wenshanensis Tsai et Liu，思茅松毛虫D. kikuchii Matsumura，赤松毛虫D. spectabilis Butler，油松毛虫D. tabulaeformis Tsai et Liu，落叶松毛虫D. superans (Butler)，云南松毛虫D. houi Lajonquiere之间的亲缘关系。13个随机引物在8种松毛虫中共检测到168个多态分子标记。分析表明，这8个种间的遗传距离的变化范围为0.3780～0.7360；马尾松毛虫与其亚种德昌松毛虫的遗传距离最近，为0.3780，与其另一亚种文山松毛虫以及油松毛虫的遗传距离次之，皆为0.5233；赤松毛虫、落叶松毛虫、云南松毛虫与马尾松毛虫的遗传距离则再次之，分别为0.6362，0.6770和0.6944；与马尾松毛虫遗传距离最远的是思茅松毛虫，为0.7360。8种松毛虫间具体的亲缘关系为: (D. superans (D. tabulaeformis (D. p. wenshanensis (D. p. tehchangensis, D. p. punctatus)(D. Kikuchii (D. spectabilis, D. houi))。

DNA fingerprinting evidence for the phylogenetic relationship of eight species and subspecies of Dendrolimus (Lepidoptera:Lasiocampidae) in China

Eight species and subspecies of Dendrolimus, D. punctatus punctatus (Walker)，D. punctatus tehchangensis Tsai et Liu，D. Punctatus wenshanensis Tsai et Liu，D. kikuchiI Matsumura，D. spectabilis Butler，D. tabulaeformis Tsai et Liu，D. superans (Butler)，and D. houi Lajonquiere have been the most serious and economically damaging insect pests in Chinese pine forests in recent years. Twenty 10-oligonucliotide primers were initially screened. We used 13 primers, which produced 168 polymorphic molecular markers to analyze the phylogenetic relationship of the eight taxa of Dendrolimus in China. The pair-wise Nei's genetic distances (Nei's D) were estimated for the eight taxa of Dendrolimus. The pairwise Nei's D varied in the range of 0.3780－0.7360. D. p. tehchangensis Tsai et Liu, and D. p. wenshanensis Tsai et Liu，which are two subspecies of D. punctatus traditionally, have nearer genetic distances with D. p. punctatus. The detailed phylogenetic relationship among the eight species and subspecies of Dendrolimus based on UPGMA should be as the following: (D. superans (D.tabulaeformis (D. wenshanensis (D. tehchangensis, D. punctatus)(D. kikuchii (D. spectabilis, D. houi)).