New Plasmid System To Select for Saccharomyces cerevisiae Purine-Cytosine Permease Affinity Mutants |
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| Authors: | Renaud Wagner Marie-Laure Straub Jean-Luc Souciet Serge Potier and Jacky de Montigny |
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| Institution: | Laboratoire de Microbiologie et de Génétique, ESA 7010 Université Louis Pasteur/CNRS, Institut de Botanique, F-67083 Strasbourg Cedex, France. |
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| Abstract: | The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function. |
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