Optimization of HIV-1 infectivity assays |
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Authors: | Jones Jennifer Whitford William Wagner Frederic Kutsch Olaf |
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Institution: | University of Alabama at Birmingham, Birmingham, AL 35294, USA. |
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Abstract: | HIV-1 reporter cell lines are the backbone of diagnostic assays, vaccine and drug development efforts. Performing HIV-1 infection experiments in a T cell background is desirable for many reasons. However, a low susceptibility to infection with primary patient isolates in available reporter T cell lines has limited such efforts. We here demonstrate that optimization of HIV-1 receptor expression and the utilization of serum free medium compositions can increase susceptibility of reporter T cell lines to HIV-1 infection by up to two orders of magnitude. |
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