Synergistic enhancement of protoplast growth by oxygenated perfluorocarbon and Pluronic F-68 |
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Authors: | P Anthony M R Davey J B Power C Washington K C Lowe |
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Institution: | (1) Department of Life Science, University of Nottingham, NG7 2RD University Park, Nottingham, UK;(2) Department of Pharmaceutical Sciences, University of Nottingham, NG7 2RD University Park, Nottingham, UK |
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Abstract: | Summary Cell suspension-derived protoplasts of albino Petunia hybrida were grown for 10 d at the interface between aqueous culture medium (KM8P) and an oxygenated (10 mbar for 15 min) perfluorocarbon liquid, perfluorodecalin. Protoplasts synthesised new cell walls and divided normally at the perfluorodecalin/culture medium interface, with a mean viability after 10 d of > 92.0%. The mean plating efficiency of protoplasts was elevated by 37% (P<0.05) following culture at the perfluorodecalin/medium interface, but was unaltered by perfluorodecalin or oxygen separately. The mean plating efficiency of protoplasts cultured at the interface was further increased to a maximium of 52% above control, in the presence of oxygenated perfluorodecalin and KM8P medium supplemented with the non-ionic, co-polymer surfactant, Pluronic F-68 at 0.01% (w/v). These findings demonstrate the effectiveness of oxygenated perfluorodecalin for promoting protoplast growth, by facilitating oxygen delivery. The finding that Pluronic F-68 further increased the plating efficiency of protoplasts cultured at the perfluorocarbon/aqueous interface suggests that these agents improve growth through separate, but cumulative, mechanisms.Abbreviations ATP
adenosine triphosphate
- PFCs
perfluorochemicals
- STP
standard temperature and pressure |
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Keywords: | Petunia hybrida protoplasts oxygen delivery perfluorochemicals Pluronic F-68 surfactant cell division |
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