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| 原生质体再生对糙皮侧耳Pleurotus ostreatus病毒脱除的效果 | |
| 引用本文： | 关园园,周素静,王震,麻兵继,高玉千,邱立友,戚元成,申进文. 原生质体再生对糙皮侧耳Pleurotus ostreatus病毒脱除的效果[J]. 菌物学报, 2011, 30(1): 108-115 |
| 作者姓名： | 关园园 周素静 王震 麻兵继 高玉千 邱立友 戚元成 申进文 |
| 作者单位： | 1. 河南农业大学生命科学学院,郑州,450002 2. 驻马店市农业科学研究所,驻马店,463000 3. 河南农业大学农学院,郑州,450002 |
| 基金项目： | 国家现代农业产业技术体系建设专项资金 |
| 摘 要： | 以糙皮侧耳Pleurotus ostreatus菌株新831和豫6为材料，从这两个菌株的菌丝体中提取了糙皮侧耳病毒基因组，共有3个dsRNA片段，大小分别为8.2kb、2.6kb和1.1kb。采用菌丝尖端分离脱毒、原基组织分离脱毒和原生质体再生脱毒技术对糙皮侧耳菌丝体进行脱毒处理，利用dsRNA技术对脱毒效果进行检测。结果显示，原基组织分离脱毒后3个条带依然存在；菌丝尖端分离脱毒后，8.2kb和1.1kb 2个条带完全脱除，2.6kb的条带亮度有所减弱；原生质体再生脱毒后3个条带完全脱除；对3种脱毒技术得到的菌株进行生理生化指标测定，结果显示，原生质体再生脱毒菌株的菌丝生长速度、生物量、呼吸强度、纤维素酶活等均明显优于出发菌株、原基组织分离脱毒和菌丝尖端分离脱毒菌株；栽培结果表明，原生质体再生脱毒菌株前两茬菇的生物学效率达到96.4%－99.1%，比出发菌株提高19.9%－25.4%，并且菌盖宽度和长度有所增加，表明原生质体再生技术可以有效脱除糙皮侧耳菌株病毒，提高糙皮侧耳栽培产量。 |
| 关 键 词： | 食用菌，糙皮侧耳，dsRNA技术，脱毒，脱毒菌株，生理性状，糙皮侧耳栽培 |
Effect of protoplast regeneration technology on the virus-elimination of Pleurotus ostreatus |
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| GUAN Yuan-Yuan,ZHOU Su-Jing,WANG Zhen,MA Bing-Ji,GAO Yu-Qian,QIU Li-You,QI Yuan-Cheng and SHEN Jin-Wen. Effect of protoplast regeneration technology on the virus-elimination of Pleurotus ostreatus[J]. Mycosystema, 2011, 30(1): 108-115 | |
| Authors: | GUAN Yuan-Yuan ZHOU Su-Jing WANG Zhen MA Bing-Ji GAO Yu-Qian QIU Li-You QI Yuan-Cheng SHEN Jin-Wen |
| Affiliation: | College of Life Science, Henan Agricultural University, Zhengzhou 450002, China;College of Life Science, Henan Agricultural University, Zhengzhou 450002, China;Zhumadian Institute of Agricultural Science, Zhumadian 463000, China;Agronomy College of Henan Agricultural University, Zhengzhou 450002, China;College of Life Science, Henan Agricultural University, Zhengzhou 450002, China;College of Life Science, Henan Agricultural University, Zhengzhou 450002, China;College of Life Science, Henan Agricultural University, Zhengzhou 450002, China;College of Life Science, Henan Agricultural University, Zhengzhou 450002, China |
| Abstract: | Three dsRNA bands with sizes of 8.2kb,2.6kb and 1.1kb respectively,were isolated from two Pleurotus ostreatus strains,Xin-831 and Yu-6. Three virus-eliminated methods,i.e. hyphal tips isolation virus-elimination (HTIVE),primordium tissue isolation virus-elimination (PTIVE) and protoplast regeneration virus-elimination (PRVE) were applied to prepare virus-eliminated strains,and the effect of virus-elimination was detected by dsRNA technique. The results showed that no dsRNA remained in the strains by PRVE me... |
| Keywords: | edible fungi Pleurotus ostreatus dsRNA technique virus-elimination virus-free strains physiological characteristics cultivation of Pleurotus ostreatus |
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