腺病毒4型E3区核苷酸序列测定及其基因结构与功能分析

本文将Ａｄ４基因组相当于７３．３－８９．２基因图谱单位的ＤＮＡ片段进行了序列测定及基因结构分析，它包括了Ａｄ４Ｅ３区全基因及该区两侧的部分序列。序列分析表明，Ａｄ４ Ｅ３区从ＴＡＴＡＡ ｂｏｘ起至该区基因结束共４７７８ｂｐ，编码１１个大于６ｋＤ的开放读码框架。对Ａｄ４ Ｅ３区ＯＲＦ分析结果表明，Ａｄ４ Ｅ３区编码的１９．３ｋ，１５ｋ，１０．４ｋ蛋白，分别与Ａｄ２ Ｅ３区的ｇｐ１９ｋ，１４．ｋ和１０

NUCLEOTIDE SEQUENCE AND STRUCTURE FUNCTIONANALYSIS OF COMPLETE E3 REGION OF ADENOVIRUS TYPE 4

he DNA fragment of Ad4 genome which ranges from 73.3m.u.to 89.2m.u.including the whole of Ad4 E3 region and part of the flanking regions has been sequenced.The sequenceanalysis showed that there are a total of 4,778 base pairs in the Ad4 E3 region,calculated from the beginning of TATAA box to the end of E3b poly A site,in which 11 open readingframes(ORFs)coding for protiens with molecular weights over 6kd are located.An analysis of ORFs indcated that the ORFs of Ad4 E3 code for proteins of 19.3kD,15KD and 10.4kD,corresponding to proteins of gp 19kD,14.7kD and 10.4kD of Ad2 E3,respectively. The E3region of Ad4 shares comparatively high nucleotide homology with those of Ad2 and Ad3,50-55%and 55-60% respectively,much higher than the homology of the whole genome of Ad4 with those of other subgroups (4-23%).Although the E3 region is nonessential for viral replication in cell culture,the facts that E3 regions are maintained in various types of adenoviruses and possess similar gene structure and high homology indicate that the E3 region has very important functions in virus infection.