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Isolation and characterization of free glycans of the oligomannoside type from the extracellular medium of a plant cell suspension |
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| Authors: | Bernard Priem Julien Solokwan Jean-Michel Wieruszeski Gérard Strecker Hassan Nazih Henri Morvan |
| Institution: | (1) Equipe Polysaccharides Pariétaux des Végétaux, Université des Sciences et Techniques de Lille Flandres-Artois, U.F.R. de Biologie, Bât. SN2, 59655 Villeneuve d'Ascq Cédex, France;(2) Laboratoire de Chimie-Biologique (Unité Mixte de Recherche du C. N. R. S. no. 111), Université des Sciences et Techniques de Lille Flandres-Artois, Bât. C9, 59655 Villeneuve d'Ascq Cédex, France |
| Abstract: | The oligosaccharides Man5GlcNAc and Man3(Xyl)GlcNAc(Fuc)GlcNAc presumed to originate fromN-glycosyl proteins have been purified from an extracellular medium (concentration: 2–5 mg/l of 14 day cultures) of white campion (Silene alba) suspension culture. Their primary structures have been determined by1H-400-MHz NMR spectroscopy and FAB-MS spectrometry. They are probably the result of an autophagic process including protein catabolism due to sucrose starvation. Additional identification of digalactosylglycerol (galactolipid breakdown) argues for this hypothesis.Abbreviations Fuc l-fucose - Man d-mannose - Xyl d-xylose - GlcNAc N-acetyl-d-glucosamine - Gal d-galactose - Glc d-glucose - FAB-MS fast atom bombardment mass spectrometry - NMR nuclear magnetic resonance |
| Keywords: | glycoconjugate oligosaccharide glycan white campion |
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