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Effect of fermentation variables on cellulase production by Trichoderma Sp.
Authors:S N Mukhopadhyay  T K Ghose  A Fiechter
Institution:(1) Indian Institute of Technology, Biochem.Engg.Research Centre, Hauz Khas, 110029 New Delhi, India;(2) ETH, Weinbergstrasse 38, CH 8092 Zurich, Switzerland
Abstract:Summary Batch cultivation ofTrichodermma reesei QM9414 was carried out in Mandels medium containing(w/v) 1% beech wood cellulose and 0.05% yeast extract at 29°C. Use of 36 hours old inoculum(10% v/v),3.2 1/min aeration rate at 400 rpm(KLa 220/h) and pH cycling strategy produced 4 g/1 cell mass and 21.5 IU/1/h FPA cellulase.
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