Effect of oxygen and peroxide on Bacteroides fragilis cell and phage survival after treatment with DNA damaging agents |
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| Authors: | Heide J.K. Slade David T. Jones David R. Woods |
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| Affiliation: | Council for Scientific and Industrial Research Microbial Genetics Research Unit, Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa |
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| Abstract: | Abstract Bacteroides fragilis Bf-2 cells were more sensitive to far-UV radiation, N -methyl- N '-nitrosoguanidine, ethylmethane sulphonate, acriflavine and mitomycin C under aerobic conditions than under anaerobic conditions. The opposite effect was observed with H2O2-treated cells and exposure to O2 enhanced the survival of H2O2-treated cells. Pretreatment of cells with sublethal concentrations of H2O2 also increased the survival of H2O2-treated cells. Reactivation of UV- and X-irradiated and methylmethane sulphonate and H2O2-treated phage b-1 was induced by O2 and H2O2 in B. fragilis . |
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| Keywords: | Bacteroides fragilis DNA damaging agents and survival phage reactivation oxygen hydrogen peroxide |
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