Analysis of adenine nucleotides and metabolic intermediates in mature and senescent leaf tissue |
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| Authors: | P K Macnicol |
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| Affiliation: | 1. Institute of Biomedical Sciences (ICBM), Faculty of Medicine, Universidad de Chile, Santiago, Chile;2. Institute for Research in Dental Sciences (ICOD), Faculty of Dentistry, Universidad de Chile, Santiago, Chile;3. Center for Regenerative Medicine, School of Medicine, Clínica Alemana-Universidad del Desarrollo, Santiago, Chile;4. Department of Toxicological and Pharmacological Chemistry, Faculty of Chemical and Pharmaceutical Sciences, Universidad de Chile, Santiago, Chile;1. MOE Key Laboratory of Geotechnical and Underground Engineering, Department of Geotechnical Engineering, Tongji University, Shanghai 200092, PR China;2. Anhui Transport Consulting and Design Institute Co., Ltd, Hefei 230088, PR China;3. Laboratory Navier/CERMES, Ecole des Ponts-ParisTech, Marne-la-Vallée 77455, France |
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| Abstract: | A rapid procedure is described for the preparation of extracts suitable for the enzymic-fluorometric analysis of adenine nucleotides and glycolytic and other phosphorylated intermediates, as well as citrate, in small samples taken from mature and senescent tobacco leaves. High recoveries of added adenine nucleotides, citrate, phosphoenolpyruvate, inorganic orthophosphate, and fructose diphosphate indicated that there were insignificant losses during extraction and that interfering substances were removed. The method utilizes extraction with trichloroacetic acid, removal of the latter with ether, and pretreatment of the resulting extract with polyvinylpyrrolidone and DEAE-cellulose. Extensions of the procedure to potato tuber tissue and to the preparation of extracts for chromatography are described. |
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