Segregation of T-DNA copies in the progeny of a regenerant plant from a mannopine-positive hairy root line |
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Authors: | C. David J. Tempé |
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Affiliation: | (1) Groupe de recherche sur les interactions entre microorganismes et plantes, Génétique et amélioration des plantes, INRA, Bat.409, F-91405 Orsay Cedex 05, France;(2) Institut de Microbiologie, UA 136, CNRS Faculté des sciences, Bat.409, F-91405 Orsay Cedex 05, France |
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Abstract: | We have analyzed opine content, T-DNA content, and developmental features of one hairy root culture line and its progeny. Opine-positive progeny still have the hairy root phenotype and have essentially the same T-DNA structure as the parental plant. Opineless progeny do not exhibit the hairy root phenotype. Some of them do not contain any T-DNA sequences, whereas others contain only the left part of T-DNA. These results are compatible with the hypothesis that, in the hairy root line analyzed, the left part of T-DNA is integrated independently from the core T-DNA and can therefore segregate during sexual reproduction. |
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Keywords: | Agrobacterium rhizogenes hairy root mannopine Ri plasmid T-DNA |
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