Preparation and characterization of poly(D,L-lactide-co-glycolide) microspheres for controlled release of human growth hormone |
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Authors: | Yilmaz Capan Ge Jiang Stefano Giovagnoli Kyu-Heum Na Patrick P. DeLuca |
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Affiliation: | (1) Hacettepe University, Ankara, Turkey;(2) BD Technologies, 27709-2016 Research Triangle Park, NC;(3) Dept of Chemistry and Technology of Drugs, University of Perugia, 06123 Perugia, Italy;(4) Research Laboratories, Dong-A Pharm Co, Ltd, 47-5, Sanggal-ri, Kiheung-up, 449-905 Youngin-si, Kyunggi-do, Korea;(5) Faculty of Pharmaceutical Sciences, University of Kentucky College of Pharmacy, 40536-0082 Lexington, KY |
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Abstract: | The purpose of this research was to assess the physicochemical properties of a controlled release formulation of recombinant human growth hormone (rHGH) encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) composite microspheres. rHGH was loaded in poly(acryloyl hydroxyethyl) starch (acHES) microparticles, and then the protein-containing microparticles were encapsulated in the PLGA matrix by a solvent extraction/evaporation method. rHGH-loaded PLGA microspheres were also prepared using mannitol without the starch hydrogel microparticle microspheres for comparison. The detection of secondary structure changes in protein was investigated by using a Fourier Transfer Infrared (FTIR) technique. The composite microspheres were spherical in shape (44.6±2.47 μm), and the PLGA-mannitol microspheres were 39.7±2.50 μm. Drug-loading efficiency varied from 93.2% to 104%. The composite microspheres showed higher overall drug release than the PLGA/mannitol microspheres. FTIR analyses indicated good stability and structural integrity of HGH localized in the microspheres. The PLGA-acHES composite microsphere system could be useful for the controlled delivery of protein drugs. |
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Keywords: | Microspheres human growth hormone protein delivery composite microspheres |
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