A rapid PCR-aided selection of a rice line containing the Rf-1 gene which is involved in restoration of the cytoplasmic male sterility |
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| Authors: | Ichikawa Norio Kishimoto Noaki Inagaki Akiko Nakamura Atsushi Koshino Yoko Yokozeki Yumi Oka Masaaki Samoto Shiro Akagi Hiromori Higo Kenichi Shinjyo Choyu Fujimura Tatsuhito Shimada Hiroaki |
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| Affiliation: | (1) Life Science Institute, Mitsui Toatsu Chemicals, Inc., Togo 1144, Mobara, 297, Japan;(2) National Institute of Agrobiological Resources, Tsukuba, 305, Japan;(3) College of Agriculture, University of Ryukyus, Okinawa, 903-01, Japan;(4) Mitsui Plant Biotechnology Research Institute, Sengen 2-1-6, Tsukuba, 305, Japan;(5) CSIRO Division of Plant Industry, GPO Box 1600, Canerra, ACT 2601, Australia;(6) Department of Biological Science and Technology, Science University of Tokyo, Yamazaki 2641, Noda, 278, Japan |
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| Abstract: | Cytoplasmic male sterility (CMS) is widely known in higher plants, the mechanism of which is believed to involve incompatibility between nuclei and cytoplasms. In rice lines with the CMS trait, fertility is restored by the aid of a nuclear-encoded gene, Rf-1, whose locus has been determined in chromosome 10. We found a particular PCR-amplified fragment, designated fL601, that specifically amplified using the DNAs from Rf-1 lines tested as templates. RFLP mapping of the fL601 locus revealed that there are two loci for the fL601, and that both are tightly linked to the Rf-1 locus. Progeny analysis also showed high frequency of their co-segregation. Southern analysis of the genomic DNA demonstrated that the Rf-1 lines shared a unique sequence in the fL601 region. These results enabled us to construct a system for specific detection of the corresponding regions. Utilizing this detection system, we established a simple PCR-mediated selection method for the Rf-1 lines, which may facilitate the breeding for hybrid rice. |
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| Keywords: | cytoplasmic male sterility genetic diagnosis hybrid rice seeds molecular marker-assisted breeding restore gene RFLP linkage map |
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