Occurrence and toxigenicity of shape Fusarium moniliforme from freshly harvested maize ears with special references to fumonisin production in Egypt |
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Authors: | Fadl Ezzat M |
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Institution: | (1) Botany Department, Faculty of Science, Minia University, Minia, Egypt |
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Abstract: | Using the seed-plate technique, 18 different isolates of Fusarium moniliforme were isolated on pentachloronitrobenzene (PCNB)
agar medium from 18 samples of a local variety of corn collected from locations in Minia Governorate.
The isolates of F moniliforme were screened for their ability to produce fumonisins on polished rice grains using the solid
state fermentation technique. Based on thin layer chromatographic (TLC) analyses using silica gel plates, 14 of the 18 isolates
tested produced FB1 and FB2 with R
f (0.17) and (0.24), respectively.
Concentration of FB1 was estimated using high performance liquid chromatography (HPLC). Production of FB1 by the 14 isolates of F. moniliforme tested ranged from 69 to 4495 ppm indicating that mouldy corn may represent a health
hazard to consumers.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Maize F moniliforme fumonisins Egypt |
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