稀有鮈鲫HAN近交系的免疫与生化标记分析

目的 筛选稀有鮈鲫HAN近交系遗传质量检测标记.方法 采用鳞片活体移植和同工酶聚丙烯酰胺凝胶电泳法对稀有鮈鲫HAN近交系的遗传纯度进行检测.结果 在免疫标记分析中,鳞片同体移植存活率为96.7％以上,野生群体移植存活率为7.4％,而HAN系F22鳞片异体移植的成功率为80％,显著高于野生群体.在生化标记分析中,在HAN系F22中无多态性位点,不同个体的同工酶谱呈现高度一致,在野生群体中有2个多态位点即est2和est3,多态位点的比例为15.56％.结论 经过多代近亲交配,稀有鮈鲫HAN近交系生化标记基因已经纯合,鳞片异体移植存活率达到80％,表明HAN系具有较高的遗传均一性.选用鳞片的异体移植及酯酶和肌蛋白分别作为免疫和生化标记对稀有鮈鲫HAN近交系进行遗传质量检测是可行的.

Indentification of immulogical and biochemical markers in an inbred strain HAN of Gobiocypris rarus

Objective To screen markers used in genetic quality detection of inbred strain HAN of Gobiocypris rarus. Methods Living donor tranplatation of scales and polyacrylamide gel electrophoresis of isozyme were used for the genetic quality control of HAN inbred strain. Results The survival rate of allotransplanted scales in the inbred strain HAN was significantly higher （80%） than that of 7.4% in the wild population. Six isozymes presumably were encoded by 13 gene loci, 11 of which were found monomorphic in the wild population, and 2 polymorphic loci （est2, est3） with a mean proportion of 15.56%. However, no polymorphic loci was found in F22. In addition, no difference between individuals was found in the inbred stock. Conclusions After successive mating brother and sisiter, inbred stock of G. rarus has reached a high degree of genetic purity. It is feasible to perform genetic quality control of HAN inbred strain of Gobiocypris rarus by scale allotransplantation and gel eleetrophoresis on Est and Mp.