Thymidine phosphorylation in wheat: analysis of phosphate transfer from ATP to thymidine |
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Authors: | Hofman J Schwarz O J |
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Affiliation: | Department of Botany, University of Tennessee, Knoxville, Tennessee 37916. |
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Abstract: | Extracts of wheat (Triticum vulgare Vill. [Triticum aestivum L.] var. Lemhi) seedlings contain thymidine-phosphorylating activity with ATP, ADP, or AMP and nucleotide hydrolase activity (ATP → → AMP). The synthesis of [32P]dTMP exclusively from [α-32P]ATP with none detectable from [γ-32P]ATP demonstrates the absence of thymidine kinase and the presence of nucleoside phosphotransferase as the only observable thymidine-phosphorylating enzyme. |
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