|
|||||
|
|
Incorporation of Antigens into Viral Capsids Augments Immunogenicity of Adeno-Associated Virus Vector-Based Vaccines |
|
| Authors: | Jan Rybniker Angela Nowag Hanna Janicki Kai Demant Pia Hartmann Hildegard Büning |
| Affiliation: | a1st Department of Internal Medicine, Division of Infectious Diseases, University Hospital Cologne, Cologne, Germany;b1st Department of Internal Medicine, Laboratory for AAV Vector Development, University Hospital of Cologne, Cologne, Germany;cCenter for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany |
| Abstract: | Genetic modification of adeno-associated virus (AAV) capsids has previously been exploited to redirect viral tropism. Here we demonstrate that engineering of AAV capsids as scaffolds for antigen display augments antigen-specific immunogenicity. Combining antigen display with vector-mediated overexpression resulted in a single-shot prime-boost vaccine. This new class of vaccines induced immune responses significantly faster and an IgG antibody pool of higher avidity than conventional vectors, highlighting the potency of capsid modification in vaccine development. |
| Keywords: | |